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Bottom-reading is usually recommended for cell-based assay with adherent cells. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Molecular Devices multi-mode microplate reader, mod SpectraMax M5e, s/n MVE05702 [East Research Lab] Auction Details Bio-Based Mushroom Leather Manufacturing Facility. 5 mP standard deviation at 1 nM SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. for Cisbio Bioassays HTRF® technology. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Homogeneous Time-Resolved Fluorescence (HTRF) . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. 6 x 10-15. 40266 Email: [email protected]. Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The self-funded NanoRacksSpectraMax® Paradigm® Multi-Mode Detection Platform. The SpectraMax M5e possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. The following document is intended. Danaher Inc spectramax m5 m5e fluorescent plate reader Spectramax M5 M5e Fluorescent Plate Reader, supplied by Danaher Inc, used in various techniques. 72 MB. cAMP HiRange 0. For example, microplates containing different samp les can be read using the same orSpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. SpectraMax Mini: 17. Page 75. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Multimode Reader Comparison Chart. Italic and boldface type are used for emphasis. Path Check Pathlength Measurement Multimode Reader Comparison Chart. 5 Z’ factor 0. Introduction. M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. Examples: “Press carefully to engage,” “Do not. 3. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. GFP fluorescence was read on days 1, 4 and 7, using a SpectraMax M5e Microplate Reader (Molecular Devices). 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. Venue Address. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 1. It was difficult and time consuming to undertake quality, repetitive research. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. M3-M5e_manual. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Supplier: Molecular Devices. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 28 nM to 0. The Spectr. 3. The Spectr. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Proceed with the following steps using optimized settings. 2 x 10-14. ISM Equipment Classification (Group 1, Class A) This equipment is. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Products : SpectraMax M3 reader, SoftMax Pro Software, SpectraMax M5/M5e reader, SpectraMax L reader, SpectraMax M4 reader Is the Gaussia Luciferase (GLuc) Assay from New England Biolabs compatible with my plate reader? The information for the GLuc Assay Kit (Cat no. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 0001 0. The resulting purple crystals were dissolved in solubilization buffer and analyzed by spectrophotometry at 490 nm, using a reference of 650 nm in a microplate reader (SpectraMax M5e, MD, USA. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. S. 41 nM were calculated using SoftMax Pro Software. com A. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 00 USD. In the M2, M5 and Plus 384, the light. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 00 USD. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. D 1 1. 1. Ex wavelength (nm/bandwidth) 485/20. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 8. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The following document is intended to demonstrate setup of this instrument. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well NanoRacks, LLC is installing a new, reconfigured Molecular Devices’ SpectraMax M5e multi-mode microplate reader on the International Space Station to perform experiments in microgravity. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. The REF button has no valid meaning for. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. Ferguson, Ph. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1. Z1. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. The validation kit included individual tubes of diluted fluorophores: FAM, HEX, and FAM+HEX. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Upgradeable modules are also available including western blot, cell imaging, and fast. FlexStation II 384. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 1. 2 x 10-14. Figure 1. We would like to show you a description here but the site won’t allow us. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Homogeneous Time-Resolved Fluorescence (HTRF) . The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Dual monochromators for assay flexibility. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. 8 x 10-15. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax M5e reconfigured to operate comfortably in the zero-gravity environment of the space station In May 2011, Molecular Devices’ SpectraMax ® M5e became the first microplate reader in space when NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Thermo Lab Systems Multiskan Ascent 354 $1,400. Introduction. The donor and acceptor can be used to label a variety of biomolecules, for applications. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. Description 1. SpectraMax Absorbance, Fluorescence, Luminescence, and Multi-Mode Readers. The Gemini XS can read a variety of sample formats from 6- to 384-well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 1. Description 1. In this product video, see how the SpectraMax M5 quickly reads samples. 2. variety of. M5/M5e Multi-Mode Microplate Reader SpectraMax. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. HTRF assay performance on the SpectraMax M5e reader is demonstrated here with two different HTRF assay kits, cAMP dynamic 2 and IP-One. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. Like the SpectraMax M5 system, the SpectraMax M5e has primary applications for fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization,. 2222 Fifth Street. 5 mP standard deviation at 1 nM. Setup Guide On The Molecular Devices SpectraMax®SpectraMax L: 36. WARNING! The instrument weighs approximately 36 pounds. 检测模式包括: • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. SpectraMax M Series Multi-Mode Microplate Readers | MolecularSpectraMax L: 36. 6 x 10-15. 304 signal level saturation. Molecular Devices SpectraMax M5e Microplate Reader $ 26,500. The detector is a CCD array with 512 pixels in x and 512 pixels in y. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. A suspicious death, an upscale spiritual retreat, and a quartet of suspects with a motive for murder. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Labsystems Fluoroskan Ascent FL Microplate Fluorometer. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. 7°C and the bottom to 36. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 3. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. 005) Photometric Precision多功能酶标仪SpectraMax M5e. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and other niche laboratory equipment lines. Settings are for each instrument are shown in Table 1. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Paradigm: W = 21. The M5 has two PMTs. In this. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. SpectraMax® ABS Plus. book Page 1 Wednesday, July 7, 2010 2:09 PM. 92 0. 97 cAMP Detection AssayMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. SpectraMax M5e. View full details Original price $5,513. SpectraMax Injector Cartridge with SmartInject™ Technology. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Example: [Drawer]. 2 x 10-14. These robust readers have been placed in labs from Antarctica to the. UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range. After excluding the background values, the cross‐reactivity of the. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). Share Tweet Share Pin. 4, Z’ = 0. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 8 x 10-15. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Five preferred detection modes. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. edu) to be added as a trained user in iLab. The schematic of the UED setup is shown as Fig. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Results obtained with SpectraMax iD5 (red), i3x (green), and M5e (blue) readers were plotted using a 4-parameter logistic, and EC 50 values of 0. GE AKTA™ Crossflow. Devices SpectraMax M5e (Ex/Em 380/650nm) • SoftMaxPro software allows easy data analysis Real-time data analysis SoftMaxPro software allows easy viewing of oxygen consumption profiles in all wells (Fig. spr SpectraMax Plus 384. 00 - Original price $5,513. Sunnyvale, Calif. 1. . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. FP assay do not perform well with protein:protein interactions. Meso Scale Discovery 1200 Sector Imager 2400. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 5. 1. Cell monolayers were decalcified with 0. 1. M5e: W = 15. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Luciferase Detection Instrumentation Guide. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. SpectraMax M5e Multi-Mode Microplate Reader. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Following the user manual for installation, maintenance, assembly or troubleshoot is an crucial prerequisite for safety in addition to protect consumers from any liability from the manufacturer of the product for viable defects in case of an insurance claim. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59 PM. Cyclic AMP titration curves were evaluated using SpectraMax Paradigm and SpectraMax M5e instruments. The following document is intended to demonstrate setup of this instrument. 1. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The following document is intended to demonstrate setup of this instrument. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax L: 36. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 0 Application Area:. SpectraMax M5e: 118. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Download PDF. About Conquer. book Page 1 Wednesday, July 7, 2010 2:09 PM. , contact Molecular. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Optimal instrument settings are the same for all three readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 1(a). View the Molecular Devices website. SpectraMax Mini: 17. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). 4, Z’ = 0. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. FlexStation™ I. 316 grating motor driver faulty. 508/20. SpectraMax M5e Multi-Mode Microplate Reader. Features. E3300S/L) can be found on the New. Microplate Reader Shelf. Gemini XPS. The plate reader. 21 MB. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se在2011年5月,凭借其耐久性,Molecular Devices SpectraMax M5e成为了第一台进入太空的微孔板读板机。NanoRacks将它加入国际空间站中的商业硬件实验平台,首次为科学家提供了在微重力环境下进行微孔板检测的机会。Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. 00. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. 0200-7027. SpectraMax® L (software version 5. This document is provided to customers who have purchased Molecular Devices, Inc. • SpectraMax M5e Multi‐Mode Microplate Reader • FlexStation® 3 Multi‐Mode Microplate Reader Overview. 5. SpectraMax Mini: 17. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. SpectraMax Mini: 17. , contact Molecular. M3-M5e_manual. Anne T. Wide range of applications. In the M2, M5 and Plus. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. StakMax Handling Stacker Base Unit. Multimode Readers Comparison Chart. Table of Contents. British Columbia, Selkirk Mountains. Lector de microplacas M5E-SC SpectraMax M5e con contrato de servicio de 1 año - Lector de placas multimodo SpectraMax M5/M5e, dispositivos moleculares - cada uno . Molecular Devices. View the Molecular Devices website. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. It does not work as expected. Table of Contents. The donor and acceptor can be used to label a variety of biomolecules, for. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. 8 x 10-15. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. #M5e) Methods Cell preparation. HTRF is compatible with a large range of available microplate readers. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Multi-Mode Microplate Readers. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. The large analog PMT works with monochromators and is used during fluorescence. 现有酶标仪的客户基本都是需要进行数据分析,可能习惯使用Excel、Prism等软件,对我们SoftMax Pro的数据分析功能不了解,事实上大家可使用SoftMax Pro对数据进行完整处理并不需要再导入第三方. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Reference and the Water Constant methods. The chamber contains springs that position the cuvette in the proper alignment for a read. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Testing we performed: The Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader passed all tests and was found to be fully functional: Power: PASSED Initialization: PASSED Display/Buttons: PASSEDThe Challenge. 00We would like to show you a description here but the site won’t allow us. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. After The Plate Is Read, Data Will Appear In The Plate Section: 11. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. 3. book Page 1 Wednesday, July 7, 2010 2:09 PM. 0. When a feature or capability applies to only certain readers, this exception is noted. Delivers single mode reader performance in one multimode reader package. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. com A. October 2023. Plate chamber design of the SpectraMax category 1 microplate readers. 0. 1. Configuring the SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers for ValitaTiter Assays. SpectraMax Mini: 17. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well7. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Microplate Readers. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. 1. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seMolecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available) $34,000. 1. Offer Ends In: 18 Days 19: 38: 21. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax M5e: 118. 00. Setup Guide. 5 mP standard deviation at 1 nMOEM: Molecular DevicesMaterial: SpectraMax M5e Common Name: Microplate Spectrophotometer Item Weight: 36 Serial Number: MVE06577 SKU#: Inv-00352193. Ends from. You must scan the fluorochrome of interest in the SpectraMax M5 or M5e reader to determine the optimal excitation and emission wavelengths for your application. 1) SpectraMax Glo Steady Luc Reporter Assay (6. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Recommended Optics Wavelength (nm) Wavelength selection Excitation 332/9 Monochromator Emission 1 488/15. D. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9The Molecular Devices SpectraMax M5e Microplate Reader enables five modes of detection. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. 485. Measured on the SpectraMax M5e™ reader, AcGFP (jellyfish), ZsGreen (reef coral), DsRed (reef coral) fluorescent proteins expressing cells can be assayed on a single plate, down to approximately 30 cells per well (384-well format), highlighting the increased light throughput efficiency and sensitivity of the MDS Analytical Technologies. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The actual pathlength, d, of a solvent is found from the following equation: d cm. No. 5. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 1. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the. 91. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2.